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KMID : 0896220000200010253
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Journal of Daegu Health College
2000 Volume.20 No. 1 p.253 ~ p.266
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The toxin purification and isolation identification of Meloidogyne hapla toxicity Bacteria
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Lee Kwang-Bae
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Abstract
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The following is experimental result of selecting soil bacteria showing toxicity against Root-knot nematode(Meloidogyne hapla). Out of 286 strains isolated from soil, one(NC67) showing toxicity against M. hapla is selected. The selected strain(NC67) is identified of B. thuringiensis subsp, indiana. It proved out that the toxic material against M. hapla produce by NC67 strain is an exotoxin. The result of examining the existence of the extercellular toxicity product by the toxic strain(NC67) by using activated carbon column chromatography, Dowex 50W column chromatography and TLC of silica1 gel etc. proved out that it is a single material.
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